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早期氧化卵白产物(AOPP)阳性规范品&试剂盒

宣布者:艾美捷科技    宣布时候:2021-06-15     
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早期氧化卵白产物.jpg


早期氧化卵(luan)(luan)白产物(AOPP,Advanced Oxidation Protein Products)是(shi)血浆(jiang)卵(luan)(luan)白被体内活性氧(ROS)进犯后构成的氧化润色产物,是(shi)早先发明的一种具(ju)备促炎活性氧化应激(ji)目标(biao)。

 

1996年Witko-Sarsat等发明(ming)(ming)CRF病(bing)(bing)人血(xue)浆中(zhong)(zhong)(zhong)AOPP程度较着增高(gao),首要(yao)存在于白(bai)(bai)卵白(bai)(bai)中(zhong)(zhong)(zhong),特点(dian)是酸性(xing)前(qian)提(ti)下(xia)在340nm有特异接收峰。在体(ti)外用次氯酸(HOC1)与白(bai)(bai)卵白(bai)(bai)感(gan)化(hua)(hua)可获得(de)与CRF病(bing)(bing)人血(xue)浆中(zhong)(zhong)(zhong)类似的(de)(de)AOPP。中(zhong)(zhong)(zhong)性(xing)粒细胞活化(hua)(hua)时髓过(guo)氧(yang)化(hua)(hua)物酶(MPO)催化(hua)(hua)产(chan)生(sheng)的(de)(de)HOC1能够润色血(xue)浆卵白(bai)(bai)而天生(sheng)适量AOPP。AOPP在泌尿、呼吸、神经结(jie)缔构(gou)造、妇科和(he)消(xiao)化(hua)(hua)等满身多个(ge)体(ti)系相干疾(ji)病(bing)(bing)产(chan)生(sheng)、成长(zhang)中(zhong)(zhong)(zhong)的(de)(de)关(guan)头感(gan)化(hua)(hua)均已被证明(ming)(ming),其(qi)在浩繁疾(ji)病(bing)(bing)中(zhong)(zhong)(zhong)的(de)(de)病(bing)(bing)发机制也逐步了了。

 

作为专业的性命迷信医药质料供给商,Cell Biolabs中国区金牌代办署理,艾美捷科技为您保举:早期氧化卵白产物(AOPP)阳性规范品&试剂盒

【1】规范品AOPP-HSA阳性规范品,AOPP-Human Serum Albumin
(早期氧化卵白产物-人血洁白卵白)
货号STA-319 (50ul, 7.5 mg/mL AOPP-HSA)
保管前提冰袋运输;收到货后,分装并保管在-20℃,以防止屡次冷冻/冻结轮回。
【2】试剂盒早期氧化卵白产物(AOPP)检测试剂盒
OxiSelect AOPP Assay Kit
货号STA-318 (200assays)
检测方式比色法
检测规模5 - 100 uM
申明书下载
尝试道理未知的含AOPP样品或氯胺规范品起首与起头显色进程的测定反映激发剂夹杂。长久孵育后,插手停止溶液,样品和规范品能够用规范比色板读取器读取。未知样品中的AOPP含量是经由过程与预约的氯胺规范曲线停止比拟来肯定的。
试剂盒组分#第一局部#
1. Chloramine Standard
2. 2Chloramine Reaction Initiator
3. Stop Solution
4. 10X Assay Diluent
#第二局部#
1. AOPP-HSA Positive Control
保管前提收到后,将AOPP-HSA阳性规范品分装并保管在-20℃,以防止屡次冷冻/冻结轮回。将一切其余试剂盒成份保管在4℃。

* 本产物仅合用于科研用处.

 

FAQ罕见题目:

1.试(shi)剂盒(he)合用于哪些范例的(de)样品?

咱们的早(zao)期氧化(hua)卵白产物(wu)(AOPP)检测试剂盒(he)可用(yong)于(yu)检测高于(yu)检测限以上的任(ren)何样(yang)品(pin)范例,比方血清,血浆,细胞(bao)/构造裂解液(冻(dong)存样(yang)品(pin):需在(zai)-80℃保管1年之内)。

 

2.应(ying)当(dang)若何筹办裂(lie)解液样本?

咱们倡议(yi)在含(han)有卵(luan)(luan)白酶按(an)捺剂(ji)的1XPBS中经由过程超声或匀浆裂解细胞或构造,以12000g离(li)心10分钟,而后搜集(ji)上(shang)清液(ye)作(zuo)为裂解液(ye)。咱们只保举利(li)用(yong)PBS制备裂解液(ye),由于(yu)咱们不晓得裂解缓冲液(ye)是不是会(hui)破坏AOPP。卵(luan)(luan)白质(zhi)浓度(du)应经由过程卵(luan)(luan)白质(zhi)测定法(fa)肯定,比方BCA或Bradford。卵(luan)(luan)白质(zhi)浓度(du)将(jiang)取(qu)决于(yu)样品(pin),但倡议(yi)在运转尝试(shi)之前停止样品(pin)滴定以肯定任何须要(yao)的浓缩度(du),以便样品(pin)落(luo)在规范曲(qu)线内。

 

3.早(zao)期氧(yang)化卵(luan)白产物(AOPP)浓度若何展(zhan)现(xian)成果?

样品 AOPP 的浓度以氯胺单元 (uM) 表现 (可参考下方颁(ban)发文(wen)献)。

 

成果展现:

AOPP试剂盒规范曲线.jpg

AOPP-HSA阳性规范品.jpg

AOPP试剂盒规范曲线AOPP-HSA阳性规范品

 

颁发文章:

  • Nukala, S.B. et al. (2021). Protein network analyses of pulmonary endothelial cells in chronic thromboembolic pulmonary hypertension. Sci Rep. 11(1):5583. doi: 10.1038/s41598-021-85004-z (#STA-318).

  • AlMarabeh, S. et al. (2020). Chronic intermittent hypoxia impairs diuretic and natriuretic responses to volume expansion in rats with preserved low-pressure baroreflex control of the kidney. Am J Physiol Renal Physiol. doi: 10.1152/ajprenal.00377.2020 (#STA-318).

  • Xiao, L.L. et al. (2020). Using advanced oxidation protein products and ischaemia-modified albumin to monitor oxidative stress levels in patients with drug-induced liver injury. Sci Rep. 10(1):18128. doi: 10.1038/s41598-020-75141-2 (STA-318).

  • Bloomer, R.J. et al. (2020). Meta- bolic Health Outcomes Following Nine Months of Mild Caloric Restriction in Male Rats Adhering To a Western or Vegan Diet. J Altern Complement Integr Med. 6:097. doi: 10.24966/ACIM-7562/100097 (#STA-318).

  • Bigazzi, R. et al. (2019). Hypertension in High School Students: Genetic and Environmental Factors: The HYGEF Study. Hypertension. 75(1):71-78. doi: 10.1161/HYPERTENSIONAHA.119.13818 (#STA-318).

  • McAllister, M.J. et al. (2019). Acute coffee ingestion with and without medium chain triglycerides decreases blood oxidative stress markers and increases ketone levels. Can J Physiol Pharmacol. doi: 10.1139/cjpp-2019-0458 (#STA-318).

  • Ungurianu, A. et al. (2019). Preclinical and clinical results regarding the effects of a plant-based antidiabetic formulation versus well established antidiabetic molecules. Pharmacol Res. 150:104522. doi: 10.1016/j.phrs.2019.104522 (#STA-318).

  • Smith, C. et al. (2019). Chronic testosterone deprivation sensitizes the middle-aged rat brain to damaging effects of testosterone replacement. Neuroendocrinology. doi: 10.1159/000504445 (#STA-318).

  • Gryszczyńska, B. et al. (2019). Advanced Oxidation Protein Products and Carbonylated Proteins Levels in Endovascular and Open Repair of an Abdominal Aortic Aneurysm: The Effect of Pre-, Intra-, and Postoperative Treatment. BioMed Research International. 2019(7976043)1-9 pages. doi: 10.1155/2019/7976043 (#STA-318).

  • Morsy, M.D. et al. (2019). Protective effect of combined melatonin and α-tocopherol administration in spinal cord ischemia-reperfusion injury in rat. Int. J. Morphol. 37(2):428-437. doi: 10.4067/S0717-95022019000200428 (#STA-318).

  • Albatayneh, E.M. et al. (2019). Serum Oxidative-Antioxidative Status in Patients With Alkaptonuria. J Clin Med Res. 11(5):337-344. doi: 10.14740/jocmr3801 (#STA-318).

  • Shell, B. et al. (2019). Angiotensin Type 1a Receptors in the Median Preoptic Nucleus Support Intermittent Hypoxia-Induced Hypertension. Am J Physiol Regul Integr Comp Physiol. doi: 10.1152/ajpregu.00393.2018 (#STA-318).

  • Bloomer, R. et al. (2018) Chronic Marijuana Smoking Does Not Negatively Impact Select Blood Oxidative Stress Biomarkers in Young, Physically Active Men and Women. Health. 10:960-970. doi: 10.4236/health.2018.107071 (#STA-318).

  • Sighinolfi, G. et al. (2018). AB0760 Advanced oxidation protein products in serum of patients with systemic sclerosis: a possible indicator of clinical evolution. Annals of the Rheumatic Diseases. 77:1516. doi: 10.1136/annrheumdis-2018-eular.6809 (#STA-318).

  • Wilson, E.N. et al. (2018). Chronic intermittent hypoxia induces hormonal and male sexual behavioral changes: Hypoxia as an advancer of aging. Physiol Behav. 189:64-73. doi: 10.1016/j.physbeh.2018.03.007 (#STA-318).

  • Gradinaru, D. et al. (2018). Insulin-Leptin Axis, Cardiometabolic Risk and Oxidative Stress in Elderly with Metabolic Syndrome. Exp Clin Endocrinol Diabetes. doi: 10.1055/s-0043-123825 (#STA-318).

  • Hány?ová, S. et al. (2017). Elevated plasma levels of advanced oxidation protein products in Slovak multiple sclerosis patients: possible association with different disability states. Act Nerv Super Rediviva. 59(2): 45–50 (#STA-318).

  • Sun, S. et al. (2018). Advanced oxidation protein products induce S-phase arrest of hepatocytes via the ROS-dependent, β-catenin-CDK2-mediated pathway. Redox Biol. 14:338-353. doi: 10.1016/j.redox.2017.09.011 (#STA-318).

  • Snyder, B. et al. (2017). Chronic intermittent hypoxia induces oxidative stress and inflammation in brain regions associated with early-stage neurodegeneration. Physiol. Rep. doi: 10.14814/phy2.13258 (#STA-318).

  • Budzyń, M. et al. (2017). The Association of Serum Thrombomodulin with Endothelial Injuring Factors in Abdominal Aortic Aneurysm. Hindawi BioMed Research International. doi: 10.1155/2017/2791082 (#STA-318).

  • Wan, X. et al. (2016). SIRT1-PGC1a-NFkB pathway of oxidative and inflammatory stress during Trypanosoma cruzi infection: benefits of SIRT1-targeted therapy in improving heart function in Chagas disease. PLoS Pathog. doi: 10.1371/journal.ppat.1005954 (#STA-318).

  • Gradinaru, D. et al. (2016). Adiponectin: possible link between metabolic stress and oxidative stress in the elderly. Aging Clin Exp Res. doi:10.1007/s40520-016-0629-z (#STA-318).

  • Crone, L. B. et al. (2016). Impact of meal ingestion rate and caffeine coingestion on postprandial lipemia and oxidative stress following high-fat meal consumption. J Caffeine Res. doi:10.1089/jcr.2016.0004 (#STA-318).

  • Huemer, M. et al. (2016). Clinical phenotype, biochemical profile, and treatment in 19 patients with arginase 1 deficiency. J Inherit Metab Dis. doi:10.1007/s10545-016-9928-y (#STA-318).

  • Martins, L. S. et al. (2015). Advanced Glycation Endproducts (AGE) evolution after pancreas-kidney transplantation: plasmatic and cutaneous assessments. Oxid Med Cell Longev. 2189582 (#STA-318).

  • ?ari?, B. et al. (2015). Oxidative stress impact on growth hormone secretion in the eye. Croat Med J. 56:326-333 (#STA-318).

  • Bloomer, R. J. et al. (2015). Comparison of a restricted and unrestricted vegan diet plan with a restricted omnivorous diet plan on health-specific measures. Healthcare.3:544-555 (#STA-318).

  • Jung, E. et al. (2015). Gemigliptin improves renal function and attenuates podocyte injury in mice with diabetic nephropathy. Eur J Pharmacol. doi: 10.1016/j.ejphar.2015.04.055 (#STA-318).

  • Thurmond, P. et al. (2015). Structural modifications of the prostate in hypoxia, oxidative stress, and chronic ischemia. Korean J Urol. 56:187-196 (#STA-318)..

  • Xie, Z. X. et al. (2014). Effect of GABA on oxidative stress in the skeletal muscles and plasma free amino acids in mice fed high-fat diet. J Anim Physiol Anim Nutr (Berl). doi: 10.1111/jpn.12254 (#STA-318).

 

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